A two-step method to covalently bind biomolecules to group-IV semiconductors: Si(111)/1,2-epoxy-9-decene/esterase.

نویسندگان

  • Claire Jeanquartier
  • Gerburg Schider
  • Sabine Feichtenhofer
  • Helmut Schwab
  • Robert Schennach
  • Johanna Stettner
  • Adolf Winkler
  • Heidrun Gruber-Woelfler
  • Georg Schitter
  • Rafael J P Eder
  • Johannes G Khinast
چکیده

A versatile two-step method has been developed that allows linking of biomolecules covalently to hydrogen-terminated group-IV semiconductors by means of epoxy-alkenes. First, the terminal C==C double bond of the alkene forms a covalent bond with the silicon, germanium, or diamond surface by UV-mediated hydrosilylation. The terminal oxirane moiety then reacts with the biomolecule. As a model system, we investigated the attachment of an esterase B to a Si(111) surface by means of the linker molecule 1,2-epoxy-9-decene. Samples were characterized by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy. The immobilized enzyme retained its activity and exhibited good long-term stability.

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عنوان ژورنال:
  • Langmuir : the ACS journal of surfaces and colloids

دوره 24 24  شماره 

صفحات  -

تاریخ انتشار 2008